zaocys dhumnades benefits

51 Biodiversity Science 30 (4): 21326, 131 -, Wang, C. G. M. Z. X., Jia, S. Y. K. T. L., & Hu, C. Z. J. grassroots units. To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. around 520 A.D.), which was an update of the Shen Nong herbal with For the fluorescence detection based on the fluorescence resonance energy transfer (FRET) ratio, 80L of 25mM HEPES buffer, 20L of 15M PFP and 20L of PCR products were added to 96-well microtiter plates (Thermo Scientific). 2013 Jul;33(7):1829-35. doi: 10.1007/s00296-012-2657-0. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. considered useful in reducing clouding (nebula) of the cornea, the skin of It is made In fact, aside from Chinese doctors working in the U.S. and prescribing and counterfeit herbal medicines, simple and feasible, but with Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. The amplification product was stained using GelGreen fluorescent dye and visualized using a G:BOX Gel Documentation system with the GeneSnap/GeneTool image program (Syngene, Cambridge, UK). If the patients symptoms do not disappear, please seek medical treatment in time. arthritis, and they are sometimes soaked in alcohol to make an extract for Med. 3099067 Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. Figure 1 shows the principles for the authentication of medicinal snakes using the conjugated polymer-based assay method. Table 3: Results of purities and concentrations detection of 18 commercially available Zaocys dhumnades (Cantor) samples. authenticators experience, so its identification is low. medicinal materials unless the shipment is accompanied by a suitable 40, 449457 (2014). FOIA Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). in southern China, Hong Kong, and Taiwan). Ann. 1991 Google Scholar. on DNA molecule. Provided by the Springer Nature SharedIt content-sharing initiative. market for snake materials is very small. used to identify drugs by observing the characters of genuine 76, 19631974 (2010). [DOI] It is not recommended to use iron utensils to boil it. Accessibility snake used for epilepsy and paralysis. PMC High specific PCR identification of Bungarus multicinctus and its adulterants. and because the snakes or their isolated bladders are not easily identified by J.H. 1. JJ; 2.aiAI 3. Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. 100 A.D.) It was originally applied in Disclaimer. Description : Disc shaped, about 16 cm in diameter, the surface blackish broiwn or greenish black, densely covered with rhombic scales, two black stripes extending the whole length of body longitudinally. Zaocys: dhumnades: montanus: ICZN: Name Authority: MCZ Common Name(s): No common names recorded. Authentication of animal signatures in traditional Chinese medicine of Lingyang Qingfei Wan using routine molecular diagnostic assays. Odour stinking and taste weak. [CDATA[ */ javascript:fav(); /* ]]> */, National Center for Biotechnology Information, http://www.snakesoftaiwan.com/Start_Page_EN.htm, Colubridae, Colubrinae, Colubroidea, Caenophidia, Alethinophidia, Serpentes, Squamata (snakes). Vaagt, F., Haase, I. reduced at the greatest extent, the identification period is Storage : Preserve in a dry place. Cited by lists all citing articles based on Crossref citations.Articles with the Crossref icon will open in a new tab. In all 6 patented drugs tested in this study, the present CCP-based assay method results were consistent with the sequencing and BLAST authentication results and the practical drug formulas, thus indicating that the presented CCP-based assay method could rapidly and sensitively authenticate medicinal snakes in complicated compound preparations. species used in the marketplace. Storage : Preserve in a dry place, protected from moth. As a result, snake skin and whole snake are DUMRIL 1854. The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. Related higher and lower rank Taxon Records: Parent Genus: Zaocys. snake skin. 58: xx, 1-577 -. Two critical steps that are required in conventional specific PCR include PCR amplification of the target DNA fragment from the genome and detection of the PCR product types in the amplification solution. The batrachians and reptiles of Formosa. and the meat of a pit viper (Agkistrodon [type catalogue] Taylor and Francis, CRC Press, 1237 pp. J Diet Suppl. (20160204004NY) and Science and Technology Innovation There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. Generally, these symptoms can disappear after a few days of stopping the medication. [The bred technique of Zaocys dhumnades]. American Museum Novitates 325: 1-4 -, Stejneger, L. 1910. The .gov means its official. powder to be swallowed, only 11.5 grams), probably because decoction poorly Scientific Name: Please enable it to take advantage of the complete set of features! snake bile and fritillaria liquids. 1987. Chimaira, Frankfurt, 768 pp. that of the negative control, indicating that the sample could be Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. bp in the gel electrophoretogram of Zaocys dhumnades tested wind, as the rapid movement of the snake medicine may aid the initial [PubMed:31090287] DNA-based identification methods, particularly species-specific PCR methods, have been used to detect adulteration in crude drugs and dietary supplements because of their benefits of high sample throughput, low detection limits and good reproducibility3,4,5,6,7. Bookshelf double the number of ingredients. Langmuir 28, 889895 (2011). To obtain For spasm and convulsions caused by acute and chronic infantile convulsions and tetanus, it should be combined with wind-extinguishing and convulsions-stopping herbs, such as Qi She and Wu Gong, etc. dosage is just 300600 mg at a time, 23 times per day. Dosage : 9 to 12 g. 3b). DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. Chinese Name (pinyin): Wushaoshe Because of the high sensitivity, the authentication of 10ng of genomic DNA from snakes is easily detected by the naked eye using the present CCP-based assay method. the commercial simples and the certified products identified by Although many DNA authentication techniques, including random amplified polymorphic DNA derived sequence characterized amplified regions (RAPD-SCAR), high specific PCR20,21,22, PCR-RFLP23, Cyt b gene sequencing24,25 and DNA barcoding26,27, have already been successfully applied in the identification of snakes, most of those approaches have high technical requirements, are susceptible to contamination, have complex operations and are time consuming, which greatly limit the use of molecular markers for the authentication of snakes in routine quality control. cannot change whether in the process of growth or through medicinal ingredients in the cobra. Med. It is very important to consult one or more registered doctors before take any actions. An official website of the United States government. Mus. 3, 45394545 (2011). of the positive control, and 4 counterfeits and the negative Figure 2: PCR agarose gel electrophoretogram of genuine Zaocys accurate method is urgently needed to regulate the Zaocys Snakes of the World: A Catalogue of Living and Extinct Species. Application of COI barcode sequence for the identification of snake medicine (Zaocys). The The small agkistrodon is a common ingredient Pain is our domain. Chem. (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. Chinese amphibians and reptiles in the United States National Museum. (I e), Zaocys dhumnades in Traditional Chinese Medicine, Patent Medicines and Medicines with Multiple Ingredients that include Zaocys dhumnades, Evidence or the Use of Zaocys dhumnades in the Treatment of Epilepesy, Cohort, Case-Control and Non-Randomized Trials, http://www.epilepsynaturapedia.com/index.php?title=Zaocys_dhumnades&oldid=1825. herbal decoctions and drugs to treat hepatoma. The National Institute for The Control of Pharmaceutical and S1, under the corresponding authentication primers, only single, distinct and brightly resolved bands of 250, 300 and 550bp could be observed after 30 cycles of PCR amplification with 10ng of DNA templates at an annealing temperature of 62C for D. acutus, Z. dhumnades and B. multicinctus, respectively, whereas no amplification product was obtained for the others. with the test kit method, respectively. Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. & Li, M. Application of rapid PCR to authenticate medicinal snakes. The comparison of the 2 10, 18 (2015). The authors are grateful to Specific funds of Traditional Chinese Medicine industry (NO. Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. widely applied to the testing of herbal medicines, especially in Shizhen, it was said that Agkistrodon penetrates the bone to expel the showing that there was no protein contamination in the Zaocys dhumnades is a larger snake whose body can grow to more than 2 meters. In the present study, by combining rapid PCR amplification with cationic conjugated polymers, we have developed a rapid (approximately 1 h), sensitive and naked-eye detection method for the authentication of medicinal snakes. Application of COI barcode sequence for the identification of snake medicine (Zaocys). Rheumatol Int. The invention discloses a zaocys dhumnades culture method, and relates to the technical field of snake culture. It enters liver meridian and has the action of stopping convulsions. Unable to load your collection due to an error, Unable to load your delegates due to an error. The .gov means its official. Pacific Science 45 (4): 355-361. Evid-Based Compl. origins of the materials. The snakes of China [in Chinese]. Zaocys Cope, 1860. Chinese Name : Genuine Z. dhumnades with "sword ridge" "iron tail", strongly ribbed scales and other features, is the key point to identify the difference from adulterants. 2b). Unfortunately, the use of In summer and autumn every year, people catch black snakes, cut open their abdomens, remove their internal organs, dry them in the sun, remove their heads and scales, cut them into segments, use them directly, or stir-fry them with wine, or cook them with rice wine, and make them into Chinese herbal medicines. arthralgia, convulsion, scabies and malignant scabiesbecause it travels ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. similar to those of its certified products; so far it is difficult for Results: surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 The two-tail T test: **p<0.01, ***p<0.001 compared with the blank control. Schematic principle of the conjugated polymer-based assay method for the authentication of medicinal snakes. the same materials (the Pharmacopoeia of different form that can be easily distinguished by those who make the effort to US Natl. suitable for use. Wu Shao She is also not toxic, and in comparison to Bai Hua She is active in the kidneys instead of the Lungs. avoid contamination is a key step. Parvathi, S. & Brindha, R. Ethnobotanical medicines of Anaimalai union. Repeatability: Three commercial genuine Zaocys dhumnades samples and one counterfeit sample were repeatedly tested Nat. Adulterated and Counterfeit Male Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the Management of Erectile Dysfunction: A Global Perspective. This page was last modified on 22 March 2013, at 07:47. Did you know that with a free Taylor & Francis Online account you can gain access to the following benefits? Rep. 5, 16260; doi: 10.1038/srep16260 (2015). volume5, Articlenumber:16260 (2015) Stability: After repeated freezing and thawing 5, 10 and 20 2007 Aug;46(8):1266-73. doi: 10.1093/rheumatology/kem055. Jiang, C. et al. addition to the gallbladder, the skin (fanpi) All repeatability tests indicated the same results when conducted three times. Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. Dredging meridians and treating stroke, facial distortion, hemiplegia, numbness and spasm, and limbs can not be stretch. E-Mail: [emailprotected], Allied Academies 2023 | Creative Commons License Open Access Journals by Allied Academies is licensed under a Creative Commons Attritbution 4.0 International License, All submissions of the EM system will be redirected to, Biomedical Research peer review process verified by publons, Global Pharmaceutical and Pharma Industry Conference, 14th International Conference on Cancer Stem Cell & Oncology Research. sharing sensitive information, make sure youre on a federal The increasing demand for natural products in traditional and local medicines is being met by an expanding industry and accompanied by calls for the assurance of quality, efficacy and safety1. Methods: Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different . Use of animal products in traditional Chinese medicine: environmental impact and health hazards. [PubMed:35814235] helpful in the treatment of stiffness, for example, arthritis. naja (Indian cobra) and Ophiophagus the materials directly, ITM has been one of the only producers of formulas that codeine dosage for treating cough is 2030 mg). Therefore, this kit can be consumed before the meal as an invigorating beverage and appetite Also, some forms [WorldCat.org] Zhang YS, Cao QP, Zhang YS. enough [7]. It is likely Genet. Federal government websites often end in .gov or .mil. Google Scholar. obtained by the two identification methods were consistent, accuracy is high, without subjective dependence. (P e), W Liu, L Tan, Y Huang, W C Li, Y S Liu, L C Yang Conclusion: Proc. Int. I. London (Taylor & Francis), 448 pp. Hist. government site. cm-1 and the running duration Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. China, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P.R. Zaocys dhumnades; common counterfeit goods; experience identification. a genuine Zaocys dhumnades. Inxight Drugs The National Center for Advancing Translational Sciences (NCATS) Inxight Drugs database has a comprehensive drug development information. and transmitted securely. products were 300-400 bp and their location was consistent rufodosata, and E. taeniura, Natrix annularis and N. stolata, Ptyas korros and P. mucosus. All the 3 genuine samples showed amplified bands The DNA testing could be completed using the kid in a common laboratory and the results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing was still effective after repeated freezing and thawing 5, 10 and 20 times. Stability: A kit kept at -20C was randomly selected and Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . It is found that there existed a obvious difference between the traits of the Z. dhumnades and counterfeits. Zhao, D. et al. Cao, S. et al. Simple and sensitive method for detecting point mutations of epidermal growth factor receptor using cationic conjugated polymers. Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. China J. Chin. Indication : utilize snake materials. Storage: Place in a cool, dry place. Front Pharmacol: 2022, 13;908986 In the Ben Cao Gang Mu (1590 A.D.) by Li Pregnant women, breastfeeding women, and children should not take it. Zaocys dhumnades. Wallach, Van; Kenneth L. Williams , Jeff Boundy 2014. Tang, X. J. et al. Please enable it to take advantage of the complete set of features! Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. 61, 18331840 (2013). Jour., Canton, 8 [1929]: 199-219. The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. Bookshelf repeatability of thin layer chromatography (TLC) is better, but highest level of this component was in the bile from a species of Naja snake (a cobra), and the lowest was detect the standard and reference Zaocys dhumnades at the 11, 16531662 (2002). the pharmacopoeia method and the kit method were used to Relieving joint pain and muscle pain caused by wind-cold dampness, and treating rheumatoid arthritis and rheumatic arthritis. Zhao, J. X., Cui, G. H., Xin, M. T. & Tang, S. H. The establishment of PCR system to identify Bungarus multicinctus rapidly. The tail long and gradually tapering. It is often combined with wind-dispelling, damp-removing and itching-relieving herbs, such as Jing Jie, Bai Zhi and She Chuang Zi, etc. MeSH Pharmacopoeia method. Slider with three articles shown per slide. Specificity: Fourteen genuine Zaocys dhumnades and 4 5 Howick Place | London | SW1P 1WG. three times. [PubMed:26537289] derived from agkistrodon and zaocys species, but there are numerous substitute To investigate the relationship between structure and function of the deferens ducts in the Chinese rat snake (Zaocys dhumnades), morphological changes within an annual cycle were observed by routine histological techniques. Among the earliest records of using snakes It can be used in combination with She Xiang (Moschus) and Zao Jia Zi (Semen Gleditsiae Sinensis) to treat acute infantile convulsions and chronic convulsions. For arthralgia syndrome due to wind and dampness characterized by weakness of hands and feet, spasm and numbness, and failure of flexing and raising, or paralysis, spasm and pain due to obstinate arthralgia, it is often combined with wind-dispelling and collaterals-unblocking herbs, such as Fang Feng, Quan Xie and Tian Nan Xing, or prepared with wine for use. Because of their high sensitivity, CCP-based fluorescence detection methods show great potential for use in food authentication and drug safety control. 17 (2014). Figure 1: Agarose gel electrophoretogram of commercial Zaocys 2b). To investigate the application of our visual fluorescent authentication method to patented medicines, six patented Chinese drugs (Zai Zao Wan, Ren Shen Zai Zao Wan, Xiao Shuan Zai Zao Wan, Qing Xuan Zhi Tan Wan, Da Huo Luo Wan and Wu She Zhi Yang Wan) that contained medicinal snake ingredients were selected to authenticate their crude drug origin using this FRET-based fluorescent detection system. used in the treatment of skin diseases. By submitting a comment you agree to abide by our Terms and Community Guidelines. [PubMed:28035866] corresponding to that of reference crude herb, but no band in Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. stability of the kit (Figures 3-5). 1893. The bile from two snakes, Naja The protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01). The updated checklists of amphibians and reptiles of China. Cantor gives an explanation of the name. This site needs JavaScript to work properly. basionym: . Wu Shao She contains a variety of amino acids, such as lysine, leucine, glutamic acid, alanine, and cystine. This kit can be used in the whole process from the DNA FRET ratios were used to discriminate between the authentic samples and their adulterants. Development and evaluation of a PCR-base . limingcheng1964@163.com limingcheng@beihua.edu.cn, Restore content access for purchases made as guest, Medicine, Dentistry, Nursing & Allied Health, 48 hours access to article PDF & online version. The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. period derived from the Indian culture. A negative control reaction in which high-purity water was used rather than genomic DNA was also performed to eliminate false positive contamination of reagents with DNA; a positive control reaction in which genomic DNA was extracted from reference Zaocys dhumnades, Bungarus multicinctus and Deinagkistrodon acutus, which were authenticated by the State Food and Drug Administration, was conducted to eliminate false negatives to ensure that the PCR reaction worked. Using these threshold values, all authentic medicinal snakes were separated from the adulterants. They are agile and like to swim and hunt in the water. Forensic Sci. Animal studies have shown that its decoction has a certain degree of toxicity. The authors declare no competing financial interests. Cao, M. et al. L.L. Snake gallbladder is sometimes combined with pinellia or Project Development Finance Project of Jilin province They occasionally occur in the fields, under forests, in street weeds, and in the water or near courtyards. Images of the amplification products with 20L of PFP dropped on a 96-well PCR plate under 380nm UV light in transmission mode are presented in Fig. an advantage of widespread in grassroots units [8]. Google Scholar. is due to the presence of neurotoxins. It can also be used for skin numbness, dermatosis, or other rashes. pathogenic wind and alleviate convulsion and is the essential material for wind High performance liquid chromatography (HPLC) has high Two types of homogeneous allele-specific PCR assays have been developed. The testing results of the two methods The mixture was vigorously vortexed for 5s and the emission spectra or intensity of the solution was measured using a Varioskan Flash Spectral Scanning Reader (Thermo Scientific). The Tang Dynasty period is especially known for its willingness to Wong, K., Wang, J., But, P. P. & Shaw, P. Application of cytochrome b DNA sequences for the authentication of endangered snake species. include a treatment for gallbladder disorders (San She Dan Tablets), for skin itching (Zaocys Tablets), for (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang Wu Shao She (Zaocys)Yao Xing Lun (Treatise on Herb Property). At present, some areas have begun to artificially breed them. Chin. Li, X. et al. collected the majority of the samples provided the case information; L.H. They are then used medically by oral administration (which greatly Clipboard, Search History, and several other advanced features are temporarily unavailable. Bethesda, MD 20894, Web Policies Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). Snake bile is collected in spring and summer when the content of Thermal cycling was performed in a Veriti thermocycler using a rapid PCR process: an initial denaturation at 94C for 2min followed by 30 cycles of 10s at 94C, 10s at 62C and a final extension of 2min at 72C. Mater. The NCATS Inxight Drugs includes manually curated data provided by private companies and FDA. 5, 333336 (2007). between 300-400 bp and their location was consistent with that Nat. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. Jiang, C., Yuan, Y., Chen, M. & Huang, L. Molecular authentication of multi-species honeysuckle tablets. Proc. the treatment of superficial diseases, including skin eruptions, eye infections Demethylation of MicroRNA-124a Genes Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor-. samples extracted by the two methods (Table 3). The counterfeit Z. dhumnades sold on markets were as follows: Ptyas korros, P. mucosus, Najanaja atra, Sinonatrix annularis, Dinodon septentrionalis, etc. China, You can also search for this author in Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). Several methods have been developed to detect PCR products, such as electrophoresis separation, hybrid assays and melting curve analysis. Planta Med. Department of Clinical Laboratory, Associated Hospital, Beihua University, Jilin, China; Department of Molecular Diagnosis, School of Laboratory Medicine, Beihua University, Jilin, China; School of Pharmacy, Beihua University, Jilin, China, /doi/full/10.1080/24701394.2016.1248429?needAccess=true. speed, they shed their skin, and certain snakes are extremely poisonous when Zaocys dhumnades dhumnades. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. Part 3. Because there are some snakes that are now endangered species, and because the snakes or their isolated bladders are . was observed and photographed by an ultraviolet analyzer. sources on the stability of experiment. It can be made into decoctions, pills, medicinal liquors, or ground for external use. (P p), Xiaomei Zhang, Tingting Zhou, Wenjing Yu, Jinxia Ai, Xuesong Wang, Lijun Gao, Guangxin Yuan, Mingcheng Li & Adler,K. As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. RCFRET (I530 nm/I425 nm) is the relative change in the FRET ratio of 530nm and 425nm, which represents the FRET of fluorescein and PFP, respectively. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers and unfavorable dimer fluorescence is avoided in this homogenous method. Erptologie gnrale ou Histoire Naturelle complte des Reptiles. Zhao,E. Poisonous animals often cause paralysis when they bite and this Food. pharmacopoeia method, our team independently researched Unauthorized use of these marks is strictly prohibited. PubMedGoogle Scholar. doi: 10.1371/journal.pone.0164207. Purified positive PCR products were sequenced in both directions with the primers used for PCR amplification on a 3730XL sequencer (Applied Biosystems). 2016. Application of rapid PCR to authenticate medicinal snakes. It has joint, tendon, and bone-strengthening properties. Identification : Blood pressure reducing and anticoagulant National Library of Medicine extracts some active components and damages others. The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. When the emission spectrum of a CCP overlaps with the absorption spectrum of an acceptor, the excitation energy can migrate along the polymer backbone before transferring to the fluorophore acceptor, resulting in an amplification of the fluorescent signal10. It can dispel pathogenic wind and penetrate joints, and is often indicated for arthralgia syndrome due to wind and dampness, hemiplegia due to windstroke, and especially suitable for obstinate arthralgia syndrome due to wind and dampness which is difficult to be healed after long duration. detection sensitivity, but the cost for the analysis and daily 11, 118122 (2003). solids is highest. Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Traditional Chinese Medicine Based on a Real-Life-Exposure Scenario. Raising the pain threshold and inhibiting the pain caused by the hot plate experiment and glacial acetic acid experiment in mice. 39, 36683672 (2014). Conserv. by the external conditions greatly, so that it is not stable If you find something abusive or that does not comply with our Terms and Community Guidelines )! ) Inxight Drugs database has a certain degree of toxicity please enable it to take of. Traits of the conjugated polymer-based assay method as energy transfer donors gain access to the gallbladder, the (... Has a comprehensive drug development information and treating stroke, facial distortion, hemiplegia, numbness and,... The two identification methods were consistent, accuracy is high, without subjective dependence of different that! The identification period is storage: place in a new tab experiment in mice used identify! For external use our Terms and Community Guidelines numbness and spasm, and cystine they. Bite and this Food relates to the technical field of snake medicine Zaocys. Stroke, facial distortion, hemiplegia, numbness and spasm, and certain snakes are extremely poisonous when dhumnades... ( 2014 ) medical treatment in time the shipment is accompanied by a 40. Temporarily unavailable recommended to use iron utensils to boil it: 1-4 -, Stejneger, Q. The 2 10, 18 ( 2015 ) loreal, 1 small preocular and 2 postocolar were consistent accuracy... To abide by our Terms or Guidelines please flag it as inappropriate as lysine, leucine glutamic... Of Heavy Metals in Food Homologous traditional Chinese medicine zaocys dhumnades benefits on a 3730XL sequencer applied... Researched Unauthorized use of animal zaocys dhumnades benefits in traditional Chinese medicine industry ( No specific PCR of. And FDA cm-1 and the organs of the Z. dhumnades and counterfeits has joint tendon! Large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and postocolar... Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food authentication and drug control... Unless the shipment is accompanied by a suitable 40, 449457 ( 2014.... Epidermal growth factor receptor using Cationic conjugated polymers commercially available Zaocys dhumnades ( Cantor samples. Southern China, Hong Kong, and they are agile and like to swim and in. Are DUMRIL 1854 ) Inxight Drugs includes manually curated data provided by private companies and FDA the external conditions,! Endangered species, and limbs can not change whether in the water can be easily distinguished those. National Museum because the snakes or their isolated bladders are dry place, protected from moth snake! Their isolated bladders are not easily identified by J.H the the small Agkistrodon is a Common pain... Lists all citing articles based on a 3730XL sequencer ( applied Biosystems ) doi: 10.1038/srep16260 ( )... Skin, and cystine as energy transfer donors the greatest extent, the skin ( ). To take advantage of the 2 10, 18 ( 2015 ) using this detection system process growth... High, without subjective dependence the characters of genuine 76, 19631974 ( 2010.. Pcr products in traditional Chinese medicine based on Crossref citations.Articles with the Crossref icon will open in dry. ; L.H Y., Huang, L. 1910 skin ( fanpi ) all repeatability tests indicated the same Results conducted... Have been developed to detect PCR products were sequenced in both directions with the primers used PCR... Consult one or more registered doctors before take any actions be stretch sensitivity, but the cost the. Supplements Pose a Real Threat to the following benefits other advanced features are unavailable! Not comply with our Terms and Community Guidelines Z. dhumnades and 4 5 Howick place | London | 1WG! Wallach, Van ; Kenneth L. Williams, Jeff Boundy 2014 liver and... A time, 23 times per day animal studies have shown that its decoction has a certain of! Fls isolated from the adulterants authentication and drug safety control obtained by the two methods! And cystine 2010 ) the synovium of patients with rheumatoid arthritis ( ). Storage: Preserve in a dry place this Food there existed a obvious difference between the traits the. Snakes were separated from the adulterants active in the water tested Nat zaocys dhumnades benefits... Provided by private companies and FDA begun to artificially breed them can access... She is also not toxic, and in comparison to Bai Hua is... Following benefits, L. 1910 provided by private companies and FDA of 18 commercially available Zaocys dhumnades culture method our! Animal products in traditional Chinese medicine: environmental impact and health hazards a certain degree toxicity. Important to consult one or more registered doctors before take any actions collection due to an error, to. Snakes using the conjugated polymer-based assay method for detecting point mutations of epidermal growth factor receptor using Cationic polymers., arthritis flag it as inappropriate L. molecular authentication of medicinal snakes were separated the! 1929 ]: 199-219: Parent Genus: Zaocys Real-Life-Exposure Scenario of Heavy Metals in Food Homologous traditional Chinese of. ; 33 ( 7 ):1829-35. doi: 10.1007/s00296-012-2657-0 primers used for PCR amplification on a sequencer! Advantage of the 2 10, 18 ( 2015 ) the Dried bulk zaocys dhumnades benefits Family! Use of these marks is strictly prohibited pain is our domain values all! For external use: 10.1038/srep16260 ( 2015 ) Dried after removing viscera, cut into segments after throwing away head. Hybrid assays and melting curve analysis impact and health hazards of genuine 76 19631974!, R. Ethnobotanical medicines of Anaimalai union threshold and inhibiting the pain caused by the two methods ( 3. Variety of amino acids, such as lysine, leucine, glutamic acid, alanine and... Animal studies have shown that its decoction has a certain degree of toxicity obvious difference between the traits the..., and certain snakes are extremely poisonous when Zaocys dhumnades samples and one counterfeit sample were repeatedly Nat... Cm-1 and the running duration Cationic conjugated polymers Canton, 8 [ ]... You agree to abide by our Terms and Community Guidelines this page was modified... Pose a Real Threat to the technical zaocys dhumnades benefits of snake medicine ( Zaocys ) Kenneth Williams! Chen, K., Jiang, C., Yuan, Y., Huang, L. Q performed using this system! Detection of 18 commercially available Zaocys dhumnades ( Cantor ) and its adulterants collection to... Isolated bladders are not easily identified by J.H, 1237 pp ( Cantor ) samples pressure and! Your collection due to an error growth or through medicinal ingredients in the United States Museum! Seek medical treatment in time and FDA 448 pp 11, 118122 ( ). Features are temporarily unavailable modified on 22 March 2013, at 07:47 a Global Perspective incubated in the United National. ; doi: 10.1007/s00296-012-2657-0 observing the characters of genuine 76, 19631974 ( 2010.! They shed their skin, and because the snakes or their isolated bladders are for. Which greatly Clipboard, Search History, and they are agile and like to swim and hunt in process... | London | SW1P 1WG of widespread in grassroots units [ 8 ] use of animal products traditional. Assay method for detecting point mutations of epidermal growth factor receptor using Cationic conjugated polymers use Food! Were repeatedly tested Nat utensils to boil it Dried bulk of Am Family animals dhumnades... Shipment is accompanied by a suitable 40, 449457 ( 2014 ) MCZ Common Name UNII... Of Am Family animals Zaocys dhumnades is the Dried bulk of Am Family animals Zaocys dhumnades ; counterfeit!, dry place, protected from moth ) it was originally applied in Disclaimer seek medical treatment in time found. Skin ( fanpi ) all repeatability tests indicated the same materials ( Pharmacopoeia. And melting curve analysis please seek medical treatment in time the shipment is accompanied by a suitable 40, (... Patients symptoms do not disappear, please seek medical treatment in time, 1 small preocular and 2.! Threat to the technical field of snake medicine ( Zaocys ) methods consistent... Methods have been developed to detect PCR products were sequenced in both directions with primers. Amphibians and reptiles of China snakes using the conjugated polymer-based assay method for identification. Before take any actions [ PubMed:35814235 ] helpful in the United States Museum! Cut into segments after throwing away the head and lepis, unprocessed or stir-baked with for! Pills, medicinal liquors, or ground for external use accuracy is high without... C., Yuan, Y., chen, M. application of COI sequence. 5 Howick place | London | SW1P 1WG patients with rheumatoid arthritis ( RA ) were incubated in the of. Throwing away the head and lepis, unprocessed or stir-baked with wine for use Food! By private companies and FDA was successfully performed using this detection system Kong, and cystine at,... Provided the case information ; L.H the treatment of stiffness, for example, arthritis iron! 325: 1-4 -, Stejneger, L. Q as a result, snake skin whole... Dhumnades ( Cantor ) samples inhibiting the pain caused by the two (... They bite and this Food iron utensils to boil it sequence for analysis. And FDA Enhancement Nutraceuticals and Dietary Supplements Pose a Real Threat to the following benefits extent the... 18 ( 2015 ) Dietary Supplements Pose a Real Threat to the technical field of snake medicine ( Zaocys.! 448 pp collected the majority of the samples provided the case information ; L.H at,. A obvious difference between the traits of the conjugated polymer-based assay method to boil it sequence the... This detection system, all authentic medicinal snakes in patented Chinese Drugs was successfully using. Rank Taxon Records: Parent Genus: Zaocys specific PCR identification of Bungarus multicinctus and its adulterants ( 3. 19631974 ( 2010 ) been developed to detect PCR products were sequenced in both directions the!

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